Betaine improves the PCR amplification of GC-rich DNA sequences.
نویسندگان
چکیده
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.
منابع مشابه
Enhanced amplification of GC-rich DNA with two organic reagents.
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 25 19 شماره
صفحات -
تاریخ انتشار 1997